| 目的：探讨膀胱癌的发生与P16基因缺失的关系。方法：采用双重PCR法对31例膀胱癌组织中P16基因进行检测。结果：31例膀胱癌组织中有7例存在P16基因缺失，缺失率为22.6%。结论：P16基因缺失与某些膀胱癌的发生存在一定的联系。
The deletions of P16 gene detected in bladder cancer by duplex PCR
LI Ze-hui
(Department of Urology,the Second Affiliated Hospital to Kunming Medical College,Kunming,650101)
XU Shao-bin ZHU Jia-you
(Division of Gecomics,Institute of Medical Biology,Chinese Academy of Medical Sciences)
GUO Ping ZHANG Zhong-ji
(Department of Pathology,Kunming Medical College)
SHI Yong-gang
(Department of Urology,the First Afilliated Hospital,Kunming Medical College)
Abstract Purpose:To investigate the deletion of P16 g ene in the bladder cancer.Methods:The P16 gene was analysi s by duplex PCR in 31 bladder cancer tissue samples.Results:The gene deletions were found in seven samples.The deletion ratio of P16 gene was 22.6 per cent.Five cases had deletion in exon 2. One case had deletion in exon 1 and one had deletion in both exon 1 and exon 2.Conclusions:The results suggest that the deletion of P16 gene were associated with some bladder cancer.
Key words Bladder tumor Gene,P16 Polymeruse chain reaction Gene deletion |
